NGS of evolved T3 phage variants - SRA

SRX8326934: NGS of evolved T3 phage variants
1 ILLUMINA (Illumina HiSeq 4000) run: 1.7M spots, 456.3M bases, 87.3Mb downloads

Design: T3 phage subjected to consecutive rounds of chemical mutagenesis, and heat-selection. Each generation was amplified by inoculation of E.coli. Genomes were purified using phenol-chloroform extraction.

Submitted by: Nextbiotics Inc

Study: Directed evolution of T3 and T7 bacteriophages for improved thermostability.

PRJNA631837 • SRP261193 • All experiments • All runs

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The goals of this project are to use a novel directed evolution technique to optimize the thermostability of bacteriophages, such to improve their efficacy as antimicrobial agents. This project tracks the emergence of mutations across the genomes of the T3 and T7 bacteriophages in order to correlate such changes with improvements in their tolerance to high temperature conditions.

Sample: T3 phage, mutagenesis series 2, iteration 0

SAMN14892577 • SRS6646745 • All experiments • All runs

Organism: Enterobacteria phage T3

Library:

Name: T3s2_v00

Instrument: Illumina HiSeq 4000

Strategy: WGS

Source: GENOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 1.7M spots, 456.3M bases, 87.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR11773894	1,725,079	456.3M	87.3Mb	2020-05-11

ID:: 10830953

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